PUBLIC REFERENCE STRUCTURES FOR ALPHAGENE ORGAN BINDING TEACHING VIEWS Retrieved: 2026-10-18 These are public target/reference-scaffold coordinates, not proprietary AlphaGene designs, candidate sequences, docking poses, measured interactions or binding-site disclosures. An animation moving the target and scaffold together is illustrative, not a molecular-dynamics trajectory or a binding prediction. Files retain real protein heavy-atom coordinates (N, CA, C, O and available side chains). Missing atoms/residues are not reconstructed. Hydrogens, DNA, ligands, waters, ions and other chains are removed. Highest-occupancy alternate conformers are selected. Source residue IDs are retained; atom serials are renumbered. Experimental constructs may differ from the reviewed sequence. See sources. RCSB PDB archive data: CC0. https://www.rcsb.org/pages/policies AlphaFold DB: CC BY 4.0. https://alphafold.ebi.ac.uk/faq Credit: AlphaFold Protein Structure Database, EMBL-EBI and Google DeepMind. Jumper et al., Nature 596, 583-589 (2021). https://doi.org/10.1038/s41586-021-03819-2 AlphaFold metadata API was inaccessible during retrieval; official versioned v6 PDB file endpoints were retrieved directly and verified as PDB coordinates. target-tet3.pdb Source: RCSB PDB 4Z3C (experimental X-ray) https://files.rcsb.org/download/4Z3C.pdb UniProt: O43151 TET3 CXXC zinc-finger experimental region. Construct covers UniProt 47-98; source IDs retained. DNA and zinc omitted. 324 heavy atoms; 45 visible residues. target-crbn.pdb Source: RCSB PDB 4TZ4 (experimental X-ray) https://files.rcsb.org/download/4TZ4.pdb UniProt: Q96SW2 Cereblon experimental structured construct. PDB 48-428 maps to UniProt 47-427. DDB1, ligand and zinc omitted. 2852 heavy atoms; 361 visible residues. target-rassf7.pdb Source: AlphaFold DB AF-Q02833-F1 v6 (predicted AlphaFold monomer) https://alphafold.ebi.ac.uk/files/AF-Q02833-F1-model_v6.pdb UniProt: Q02833 RASSF7 Ras-associating domain, public predicted monomer residues 6-89. 643 heavy atoms; 84 visible residues. target-adrb1.pdb Source: AlphaFold DB AF-P08588-F1 v6 (predicted AlphaFold monomer) https://alphafold.ebi.ac.uk/files/AF-P08588-F1-model_v6.pdb UniProt: P08588 Beta-1 adrenergic receptor transmembrane region, public predicted monomer residues 54-399. Membrane and signaling partners omitted. 2702 heavy atoms; 346 visible residues. target-smad3.pdb Source: RCSB PDB 1MJS (experimental X-ray) https://files.rcsb.org/download/1MJS.pdb UniProt: P84022 SMAD3 MH2 domain experimental monomer. PDB 228-424 maps to UniProt 229-425. Unresolved residues omitted. 1397 heavy atoms; 178 visible residues. target-cda1.pdb Source: AlphaFold DB AF-Q9H2G4-F1 v6 (predicted AlphaFold monomer) https://alphafold.ebi.ac.uk/files/AF-Q9H2G4-F1-model_v6.pdb UniProt: Q9H2G4 CDA1/TSPYL2 public predicted central structural segment 210-460. This is a visual segment, not a validated binding site or precisely annotated functional-domain boundary. 2130 heavy atoms; 251 visible residues. target-stat3.pdb Source: RCSB PDB 6NJS (experimental X-ray) https://files.rcsb.org/download/6NJS.pdb UniProt: P40763 STAT3 experimental core construct, UniProt 127-688. Ligand and unresolved residues omitted; deposited residue IDs retained. 4166 heavy atoms; 523 visible residues. target-sting.pdb Source: RCSB PDB 4EMT (experimental X-ray) https://files.rcsb.org/download/4EMT.pdb UniProt: Q86WV6 STING cyclic-dinucleotide-binding domain experimental monomer; construct maps to UniProt 155-341. Source residue IDs retained, including deposited N-terminal SER154. Ligand, dimer partner and membrane region omitted. 1399 heavy atoms; 173 visible residues. target-iga1.pdb Source: RCSB PDB 1OW0 (experimental X-ray) https://files.rcsb.org/download/1OW0.pdb UniProt: P01876-1 IgA1 Fc heavy-chain constant-region segment. PDB 241-454 maps to UniProt 122-335; visible 242-450 maps to 123-331. Secreted and membrane isoforms share this region. No hinge glycans, complete antibody or Gd-IgA1 glycoform is represented. 1435 heavy atoms; 209 visible residues. scaffold-reference.pdb Source: RCSB PDB 1L2Y (experimental solution NMR; model 1) https://files.rcsb.org/download/1L2Y.pdb UniProt: Not applicable; de novo TC5b miniprotein TC5b Trp-cage 20-residue miniprotein, first NMR model only. Public reference scaffold, not a designed candidate or validated binder to these targets. 154 heavy atoms; 20 visible residues. SCAFFOLD CREDIT 1L2Y: Neidigh, Fesinmeyer and Andersen, Designing a 20-residue protein. Nature Structural Biology 9, 425-430 (2002). https://doi.org/10.1038/nsb798 https://www.rcsb.org/structure/1L2Y TC5b Trp-cage, first NMR conformer. It is not a designed or validated binder to TET3, CRBN, RASSF7, ADRB1, SMAD3, CDA1, STAT3, STING or IgA1. IgA NOTE The IgA1 asset is a public Fc fragment. Gd-IgA1 is defined by hinge glycosylation, which is not represented here. BHV-1400 is a drug candidate, not a protein or the reference scaffold supplied here.